FM drop out mixture, minus Glutamic acid and w/o Methionine, 7500 mg/l

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Description

FM drop out mixture, minus Glutamic acid and w/o Methionine, 7500 mg/lThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to

44 Agar 15 Final pH 7

5 (100 g/l) Assay ≥99%

0 (100 g/l) Assay ≥99%

The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations

0 M 242

anhydrous 0

49 Agar 17 Suspend 41

16 pH (100 g/l) 4

YEP Medium is based upon YPD but is without dextrose and can be used as a base for making YPD media with an alternate carbon source

3 g/L Store at room temperature

food production

Weight 150